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Istituto Italiano di Tecnologia - Center for Advanced Biomaterials for Health Care

Technical Contact

Annachiara Scalzone
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Instruments

Multifunctional Microscopy Unit

  • Multiphoton microscopy (two-photon/advanced confocal) for deep three-dimensional imaging of 3D neural models
  • Super-resolution microscopy (STED) for subcellular analysis of neuronal and synaptic structures
  • Advanced electron microscopy platforms (SEM; TEM; FIB-SEM) for ultrastructural characterization of bioengineered tissues

Organ on Chip

  • Customized organ-on-chip microfluidic platforms for modeling the brain microenvironment
  • Microfabrication systems for microfluidic chips; including micromilling; soft lithography and 3D printing
  • MEA systems for in vitro electrophysiological recordings from 3D neural networks integrated into organ-on-chip models

Procedures

  • Characterization of optical and optoelectronic devices with the aid of open-source/open-hardware infrastructures that allow the implementation of complex optoelectronic measurements in both the visible and infrared ranges
  • Two-photon polymerization (2PP) on the non-planar surface of a tapered optical fiber using a near-infrared laser
  • Confocal and multiphoton imaging to characterize the properties of tapered optical fibers for optogenetics and fiber photometry, allowing the measurement of both the emission and collection light fields
  • Multiphoton characterization of the optical properties of biological tissues
  • Raman and hyperspectral imaging of organic and inorganic samples
  • Fiber Raman microscopy
  • Fiber holography for endoscopic Raman measurements
  • Super-resolution imaging based on STED microscopy
  • High-sensitivity UV-VIS-NIR spectrophotometry
  • High-resolution atomic force microscopy integrated with an inverted line microscope (AFM)
  • Fabrication of optical fibers and small-diameter fused silica capillaries
  • Processing and characterization of materials through deposition processes on substrates and devices: thermal depositions of metals, AlN depositions via piezoelectric sputtering, and conformal Parylene coatings for complex 3D devices and MEMS
  • Front-end techniques for the nanofabbrication and microfabbrication of passive and active semiconductor- and polymer-based piezoelectric microelectromechanical systems (MEMS) for energy harvesting and biosensing
  • Back-end technologies useful for the second phase of MEMS and device microfabbrication: encapsulation of the individual chip within the chip carrier and the relative wiring for chip-to-package connections
  • Dual-beam FIB/SEM system: including automated slice-and-view FIB tomography, electron and ion beam lithography, nanomanipulation, in situ probing, EDS, EBSD, and a range of imaging modalities. Deposition of Pt, Au, SiOx, and etching (XeF2)
  • 3D printer systems
  • Detailed electrochemical and spectroelectrochemical characterization of semiconductor thin films and electrochemical devices using tools for identifying critical performance parameters of photovoltaic devices
  • Contact mechanics, lubrication, and theoretical tribology; experimental tribology (adhesion, friction, lubrication, wear, optical and electrical characterization of contact pairs); rheometry and spectroscopy; functionalization and chemical/physical characterization of surfaces
  • Synthesis and characterization of engineered nanomaterials for biological applications
  • Synthesis of organic molecules used as surface coatings, binders, and labels, conjugation of biomolecules, in vitro testing of materials on mammalian cell cultures and bacteria
  • High-end data processing with high computing power and fast algorithms requiring high CPU and memory utilization