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Istituto Italiano di Tecnologia - Center for Biomolecular Nanotechnologies

Technical Contact

Cinzia Montinaro
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Ferruccio Pisanello
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Instruments

Multifunctional Microscopy Unit

  • Two-photon imaging system
  • Slice scope - Confocal Spinning Disk
  • Raman microscopy system
  • Three-laser fiber photometry system (Galvo - Station)
  • Hyperspectral imaging system
  • Fiber Raman microscopy system
  • Two-photon lithography system (mesoscope)
  • Fiber holography system for endoscopic Raman measurements
  • Leica SP8 – STED confocal microscope
  • Stereomicroscope
  • Optical microscope
  • EVOS fluorescence microscope
  • TEM

Multifunctional Implantable and Wearable Tech

  • Fiber photometry system with Synapse software platform (TDT) for neural recordings

Front & Back End

  • AFM
  • FIB/SEM
  • Benchtop SEM
  • Hummink
  • Dragonfly
  • Flip-chip
  • Micro-welder
  • Pull-shear tester
  • Semiconductor analyzer
  • Probe-station with source meter
  • Climatic chamber
  • Blade dicing saw
  • Sputter coater
  • Reflow oven with screen printing for small prototypes
  • Spectrum analyzer
  • VNA
  • Thermal/e-beam evaporator for metals
  • ICP STS
  • KJL Sputtering
  • Field Flow Fractionation (FFF)
  • Laser cutter
  • Laser Doppler vibrometer
  • Parylene Coater SCS Coating
  • System for fiber and micropipette fabrication (Puller)
  • Filament 3D printers (FDM)
  • Freeze dryers
  • Glove box
  • DLS (Dynamic Light Scattering)
  • DMA
  • Atomic force microscopy
  • Rheometer
  • Contact angle
  • Stylus profilometry
  • Thorlabs-based triboscope
  • Nikon-based triboscope
  • STLA 2.5D Microfluidics/Masks
  • STLA 5 micrometers
  • STLA 15 micrometers
  • STLA 25 micrometers
  • Magnetic field generator (Electro-physical laboratory
  • Double Helmholtz)
  • Voltage Amplifiers (Glassman high voltage DC
  • Falco amplifier AC
  • DC portable amplifiers)
  • Oscilloscopes and signal generators

Cleanroom Technologies

  • Oxygen plasma
  • Obducat – Imprint lithography
  • ICP Oxford – Dry Etching
  • Profilometer
  • Exposure unit
  • Spray-coater
  • Optical microscope
  • Mask-aligner
  • Nanoscribe
  • Chemical hood for lithography
  • Chemical hood for wet-etching

Organoid on Chip

  • QuantStudioTM qPCR for Real-time PCR
  • InvitrogenTM iBrightTM Imagers for Western Blot
  • ICP – OES Agilent
  • Bacteria incubator
  • HPLC Agilent
  • Chemical hood for acids (Kottermann)
  • Chemical hood for solvents (Kottermann)
  • Elix 35 Millipore water treatment system with two dispensing stations
  • Refrigerated benchtop centrifuge (Beckman Coulter Allegra X-32 R I)
  • Refrigerated benchtop centrifuge (Beckman Coulter Allegra X-32 R II)
  • Analytical balances
  • TECAN Infinity 100 (fluorescence and luminescence reader with double monochromator)
  • X-ray diffractometer
  • Laminar flow hoods
  • Biological safety cabinet
  • Incubators
  • Nucleofector (LONZA)

Procedures

  • Characterization of optical and optoelectronic devices using open-source/open-hardware infrastructures that enable complex optoelectronic measurements in both the visible and infrared spectrum
  • Two-photon polymerization (2PP) on the non-planar surface of a tapered optical fiber using a near-infrared laser
  • Confocal and multi-photon imaging to characterize the properties of tapered optical fibers for optogenetics and fiber photometry, enabling measurement of both emission and collection light fields
  • Multi-photon characterization of the optical properties of biological tissues
  • Raman and hyperspectral imaging of organic and inorganic samples
  • Fiber Raman microscopy
  • Fiber holography for endoscopic Raman measurements
  • Super-resolution imaging based on STED microscopy
  • High-sensitivity UV-VIS-NIR spectrophotometry
  • High-resolution atomic force microscopy integrated with an inverted optical microscope (AFM)
  • Fabrication of optical fibers and small-diameter fused silica capillaries
  • Processing and characterization of materials through deposition processes on substrates and devices: thermal metal depositions, AlN depositions via piezoelectric sputtering, and conformal Parylene coatings for complex 3D devices and MEMS
  • Front-end techniques for the nanofabrication and microfabrication of passive and active piezoelectric microelectromechanical systems (MEMS) based on semiconductors and polymers for energy harvesting and biosensing
  • Back-end technologies for the second phase of MEMS and device microfabrication: single-chip encapsulation within the chip carrier and respective wire bonding for chip-to-package connections
  • Dual-beam FIB/SEM system: featuring automated slice-and-view FIB tomography, electron and ion beam lithography, nanomanipulation, in-situ probing, EDS, EBSD, and various imaging modes, along with Pt, Au, SiOx deposition and etching (XeF2)
  • 3D printer systems
  • Detailed electrochemical and spectroelectrochemical characterization of semiconductor thin films and electrochemical devices using tools for identifying critical performance parameters of photovoltaic devices
  • Contact mechanics, lubrication, and theoretical tribology; experimental tribology (adhesion, friction, lubrication, wear, optical and electrical characterization of contact pairs); rheometry and spectroscopy; functionalization and chemical/physical characterization of surfaces
  • Synthesis and characterization of engineered nanomaterials for biological applications
  • Synthesis of organic molecules used as surface coatings, linkers, and labels, biomolecule conjugation, and in-vitro testing of materials on mammalian cell cultures and bacteria
  • High-end data processing with high computational power and fast algorithms requiring intensive CPU and memory utilization